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image analyzer 2 microscope  (Carl Zeiss)


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    Carl Zeiss image analyzer 2 microscope
    Image Analyzer 2 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+analyzer+2+microscope/pm39932662-34-10-13?v=Carl+Zeiss
    Average 90 stars, based on 1 article reviews
    image analyzer 2 microscope - by Bioz Stars, 2026-07
    90/100 stars

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    (A) Isolation from liposuction sample. (B) Upper and lower left, ASCs and mature differentiated adipocytes, both stained with red oil (×100): upper left figure shows ASCs stained, negative for red oil test, and adherent in plasticware plate; lower left shows mature differentiated adipocytes stained, positive for red oil test, and adherent in plasticware plate. (B) Upper and lower right, differential interference contrast (DIC-Nomarski) combined with DAPI (×400) for ASCs and mature differentiated adipocytes is shown. (C) Mature differentiated adipocytes with red oil staining (magnification at ×100, ×200, ×400, and ×630, scale bar = 40 μm) are shown. Axioskop-2-Zeiss microscope.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Leptin and TGF-β1 Downregulate PREP1 Expression in Human Adipose-Derived Mesenchymal Stem Cells and Mature Adipocytes

    doi: 10.3389/fcell.2021.700481

    Figure Lengend Snippet: (A) Isolation from liposuction sample. (B) Upper and lower left, ASCs and mature differentiated adipocytes, both stained with red oil (×100): upper left figure shows ASCs stained, negative for red oil test, and adherent in plasticware plate; lower left shows mature differentiated adipocytes stained, positive for red oil test, and adherent in plasticware plate. (B) Upper and lower right, differential interference contrast (DIC-Nomarski) combined with DAPI (×400) for ASCs and mature differentiated adipocytes is shown. (C) Mature differentiated adipocytes with red oil staining (magnification at ×100, ×200, ×400, and ×630, scale bar = 40 μm) are shown. Axioskop-2-Zeiss microscope.

    Article Snippet: The percentage of cells undergoing adipogenesis was calculated by image analyzer microscope (Axioskop-2-Zeiss, Jena, Germany).

    Techniques: Isolation, Staining, Microscopy

    (A) Flow cytometry analysis on ASCs and on mature differentiated adipocytes for antigen surface markers: the results are shown as box-plots with medians (lines inside the boxes), mature differentiated adipocytes significantly expressed CD105, CD73, and CD90 in comparison with ASCs (* p = 0.0272 for CD105, ** p < 0.0001 for CD73, and CD90; n = 3 experiments). Analysis of variance (ANOVA), Fisher’s PLSD. Right, representative examples of flow cytometric analysis in ASCs and in adipocytes. The numbers indicate the percentage of double positive cells for CD73 and CD90. (B) Representative immunofluorescence for antigen surface markers on mature differentiated adipocytes is shown: higher, differential interference contrast (DIC-Nomarski) combined with DAPI and fluorescence; lower, DAPI with fluorescence; negative, CD34 (green), CD73 (red), and CD90 (green) (magnification at ×400, scale bar = 40 μm). Axioskop-2-Zeiss microscope.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Leptin and TGF-β1 Downregulate PREP1 Expression in Human Adipose-Derived Mesenchymal Stem Cells and Mature Adipocytes

    doi: 10.3389/fcell.2021.700481

    Figure Lengend Snippet: (A) Flow cytometry analysis on ASCs and on mature differentiated adipocytes for antigen surface markers: the results are shown as box-plots with medians (lines inside the boxes), mature differentiated adipocytes significantly expressed CD105, CD73, and CD90 in comparison with ASCs (* p = 0.0272 for CD105, ** p < 0.0001 for CD73, and CD90; n = 3 experiments). Analysis of variance (ANOVA), Fisher’s PLSD. Right, representative examples of flow cytometric analysis in ASCs and in adipocytes. The numbers indicate the percentage of double positive cells for CD73 and CD90. (B) Representative immunofluorescence for antigen surface markers on mature differentiated adipocytes is shown: higher, differential interference contrast (DIC-Nomarski) combined with DAPI and fluorescence; lower, DAPI with fluorescence; negative, CD34 (green), CD73 (red), and CD90 (green) (magnification at ×400, scale bar = 40 μm). Axioskop-2-Zeiss microscope.

    Article Snippet: The percentage of cells undergoing adipogenesis was calculated by image analyzer microscope (Axioskop-2-Zeiss, Jena, Germany).

    Techniques: Flow Cytometry, Comparison, Immunofluorescence, Fluorescence, Microscopy

    (A) Flow cytometry analysis on ASCs for PREP1: both leptin (0.05 μM) and TGF-β1 (400 nM) significantly reduced PREP1 expression (* p = 0.0149 and ** p = 0.0318, respectively); the results are shown as box-plots with medians (lines inside the boxes), 25th and 75th percentiles (limits of boxes), and the 10th and 90th percentiles (whiskers) ( n = 7 experiments). Analysis of variance (ANOVA), Fisher’s PLSD. Right, representative examples of flow cytometric analysis. The numbers indicate the percentage of positive cells for PREP1. (B) Representative immunofluorescence for PREP1 (green) on ASCs is shown: higher, differential interference contrast (DIC-Nomarski) combined with DAPI and fluorescence; lower, DAPI with fluorescence (magnification at ×400, scale bar = 40 μm). Axioskop-2-Zeiss microscope.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Leptin and TGF-β1 Downregulate PREP1 Expression in Human Adipose-Derived Mesenchymal Stem Cells and Mature Adipocytes

    doi: 10.3389/fcell.2021.700481

    Figure Lengend Snippet: (A) Flow cytometry analysis on ASCs for PREP1: both leptin (0.05 μM) and TGF-β1 (400 nM) significantly reduced PREP1 expression (* p = 0.0149 and ** p = 0.0318, respectively); the results are shown as box-plots with medians (lines inside the boxes), 25th and 75th percentiles (limits of boxes), and the 10th and 90th percentiles (whiskers) ( n = 7 experiments). Analysis of variance (ANOVA), Fisher’s PLSD. Right, representative examples of flow cytometric analysis. The numbers indicate the percentage of positive cells for PREP1. (B) Representative immunofluorescence for PREP1 (green) on ASCs is shown: higher, differential interference contrast (DIC-Nomarski) combined with DAPI and fluorescence; lower, DAPI with fluorescence (magnification at ×400, scale bar = 40 μm). Axioskop-2-Zeiss microscope.

    Article Snippet: The percentage of cells undergoing adipogenesis was calculated by image analyzer microscope (Axioskop-2-Zeiss, Jena, Germany).

    Techniques: Flow Cytometry, Expressing, Immunofluorescence, Fluorescence, Microscopy

    (A) Flow cytometry analysis on mature differentiated adipocytes for PREP1: both leptin (0.05 μM) and TGF-β1 (400 nM) significantly reduced PREP1 expression (* p = 0.0138 and ** p = 0.0461, respectively); the results are shown as box-plots with medians (lines inside the boxes), 25th and 75th percentiles (limits of boxes), and the 10th and 90th percentiles (whiskers) ( n = 6 experiments). Analysis of variance (ANOVA), Fisher’s PLSD. Right, representative examples of flow cytometric analysis. The numbers indicate the percentage of positive cells for PREP1. (B) Representative immunofluorescence for PREP1 (green) on mature differentiated adipocytes is shown: higher, differential interference contrast (DIC-Nomarski) combined with DAPI and fluorescence; lower, DAPI with fluorescence (magnification at ×400, scale bar = 40 μm). Axioskop-2-Zeiss microscope.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Leptin and TGF-β1 Downregulate PREP1 Expression in Human Adipose-Derived Mesenchymal Stem Cells and Mature Adipocytes

    doi: 10.3389/fcell.2021.700481

    Figure Lengend Snippet: (A) Flow cytometry analysis on mature differentiated adipocytes for PREP1: both leptin (0.05 μM) and TGF-β1 (400 nM) significantly reduced PREP1 expression (* p = 0.0138 and ** p = 0.0461, respectively); the results are shown as box-plots with medians (lines inside the boxes), 25th and 75th percentiles (limits of boxes), and the 10th and 90th percentiles (whiskers) ( n = 6 experiments). Analysis of variance (ANOVA), Fisher’s PLSD. Right, representative examples of flow cytometric analysis. The numbers indicate the percentage of positive cells for PREP1. (B) Representative immunofluorescence for PREP1 (green) on mature differentiated adipocytes is shown: higher, differential interference contrast (DIC-Nomarski) combined with DAPI and fluorescence; lower, DAPI with fluorescence (magnification at ×400, scale bar = 40 μm). Axioskop-2-Zeiss microscope.

    Article Snippet: The percentage of cells undergoing adipogenesis was calculated by image analyzer microscope (Axioskop-2-Zeiss, Jena, Germany).

    Techniques: Flow Cytometry, Expressing, Immunofluorescence, Fluorescence, Microscopy

    (A) Flow cytometry analysis on mature differentiated adipocytes for TLR4: both leptin (0.05 μM) and TGF-β1 (400 nM) significantly increased TLR4 expression (* p = 0.0178 and ** p = 0.0325, respectively); the results are shown as box-plots with medians (lines inside the boxes) ( n = 4 experiments). Analysis of variance (ANOVA), Fisher’s PLSD. Right, representative examples of flow cytometric analysis. The numbers indicate the percentage of positive cells for TLR4. (B) Representative immunofluorescence for TLR4 (red) on mature differentiated adipocytes is shown: higher, differential interference contrast (DIC-Nomarski) combined with DAPI and fluorescence; lower, DAPI with fluorescence (magnification at ×400, scale bar = 40 μm). Axioskop-2-Zeiss microscope.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Leptin and TGF-β1 Downregulate PREP1 Expression in Human Adipose-Derived Mesenchymal Stem Cells and Mature Adipocytes

    doi: 10.3389/fcell.2021.700481

    Figure Lengend Snippet: (A) Flow cytometry analysis on mature differentiated adipocytes for TLR4: both leptin (0.05 μM) and TGF-β1 (400 nM) significantly increased TLR4 expression (* p = 0.0178 and ** p = 0.0325, respectively); the results are shown as box-plots with medians (lines inside the boxes) ( n = 4 experiments). Analysis of variance (ANOVA), Fisher’s PLSD. Right, representative examples of flow cytometric analysis. The numbers indicate the percentage of positive cells for TLR4. (B) Representative immunofluorescence for TLR4 (red) on mature differentiated adipocytes is shown: higher, differential interference contrast (DIC-Nomarski) combined with DAPI and fluorescence; lower, DAPI with fluorescence (magnification at ×400, scale bar = 40 μm). Axioskop-2-Zeiss microscope.

    Article Snippet: The percentage of cells undergoing adipogenesis was calculated by image analyzer microscope (Axioskop-2-Zeiss, Jena, Germany).

    Techniques: Flow Cytometry, Expressing, Immunofluorescence, Fluorescence, Microscopy